Come back to fully analyzed and quantitated results in just 3 hours. Her fluorescent detection enables multiplexing, letting you maximize your time and sample. By continuing to use this website you acknowledge this and agree to our cookie policy. This protocol also describes treatment of glycosylated proteins with PNGase F. Dilute the antibodies in Antibody Diluent 2. Minneapolis, MN 55413 Copyright 2023 R&D Systems, Inc. All Rights Reserved. Do not use past expiration date. It also comes with EZ Standard Pack, 12-230 KDa, PS- ST01EZ-8 o SM-004 is the most commonly used since most proteins are ProteinSimple will continue service and support of the Wes platform through July 30th, 2026. Novus Biologicals branded products are not currently available on biotechne.com and can be found at novusbio.com. Incubation with Streptavidin-HRP followed by Luminol/Peroxide generates a chemiluminescent signal for the total captured protein. Open Jess's door. The variability in the manual processes that impact reproducibility, quantitation, time to result, and overall data reliability is eliminated. Mayo Clinic Has anyone used the ProteinSimple Wes capillary western blot system for brain tissue? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through biotechne.com. Best of all, Jesss multiple detection capabilities enable two-color protein detection for multiplexing and chemiluminescent detection, on top of protein normalization. <>/Metadata 248 0 R/ViewerPreferences 249 0 R>> Just load your samples and reagents into the microplate, and Jess does the rest. Minneapolis, MN 55413 Please use the form below to provide feedback related to the content on this product. Total protein detection happens in the same capillary on the same sample, but runs on a separate channel from NIR so you get to keep your existing IR, NIR and chemiluminescence channels. While SYPRO Ruby requires at least 1 ng of protein for reliable detection, Simple Western Total Protein Assay Methodsusing 5X Biotin Labeling Reagent can reliably detect as little as 150 pg. However, we are committed to improving your shopping experience. Meet Jess, your protein analysis problem solver. TheChemiluminescent Total Protein Assaycan be used to detect all the proteins in your lysate, similar to a Coomassie or SYRPO Ruby stained gel. PDF User Guide for Jess - Bio-Techne Jess offers market-leading protein detection sensitivity for Western blotting workflows. Jess, shes like Western blot meets ELISA in one. and how expensive is. PDF Detection Module for Jess, Abby, Wes, Chemiluminescence Peggy Sue and Head of Research Instrumentation, Cancer Research UK Cambridge Institute, "Some of the proteins we wanted to detect are low in abundance and its really hard to get a good amount of protein for traditional Western blot. USA, TEL 612 379 2956 We appreciate your feedback. With Jess's chemiluminescent detection, you'll get picogram level sensitivity letting you maximize the data you get from your sample. In just 3 hours you'll have quantitative, size-based data ready for analysis. In contrast to membrane-anchored ADAMs, ADAMTSs are secreted and are all active proteases. ", Irene Tobias, Ph.D., With Wes, we can do multiple assays with one sample collection. Bio-Techne uses cookies to provide you with a great website experience. Has anyone used the ProteinSimple Wes capillary western - ResearchGate Lane 1: ladder; Lane 2: phospho-Stat3; Lane 3: Stat 3; Lane 4: phospho-Stat3/Stat3. Assays are performed on Simon ,. Forbes D. Porter. For more information visit or contact us at: Toll-free: 888 607 9692 Tel: 408 510 5500 . Protein analysis comes with many challengeslabor-intensive protocols increase time to result and multiple hands-on steps increase . PDF Compass Software for Simple Western Size Assays Quick Reference Guide The formulation of the Stellar Total Protein Detection Module (DMTP03) is unique to Stellar fluorescence assays on Jess and is different from the Total Protein Detection Module (DM-TP01). Come back to fully analyzed western blotting results on 24 samples in 3 hours. ", Chris LaBreck, Ph.D., At best, you end up with Western blot-like, semi-quantitative results when what you really need, and deserve, is highly reproducible immunoassay quantitation. Simple Western assays are automated, capillary-based immunoassays that solve many of the challenges that come with traditional Westerns. Jess Simple Western System 004-650: R&D Systems Insert a capillary cartridge into the cartridge holder. CST Antibodies Validated for Simple Western It would take about a week to run 40 proteins using Western blotting. And obviously I like getting results the same day rather than having to wait two days", Emma Wilson (ne Humphrey), Ph.D., Chemiluminescent detection gives you picogram-level sensitivity, letting you maximize the data you get from your sample. Simple Western data are displayed by lane in a virtual blot-like image similar to traditional Western blot results. After separation & immobilization of your sample proteins, the Protein Normalization Assay Module for Simple Western uses a proprietary molecule to fluorescently label proteins in your sample for detection. Novus Biologicals branded products are not currently available on biotechne.com and can be found at novusbio.com. Assistant Professor, Womens and Childrens Health, Karolinska Institute, Stockholm, Sweden. Like multiplexed chemiluminescent and fluorescent assays on the same sample at the same time. Transform your cancer & immuno-oncology research by utilizing next-generation Western blotting tools to give you richer views into your samples. Jess reagents and consumables from ProteinSimple, a Bio-Techne brand, are designed for use with ProteinSimple's Jess, a Simple Westernplatform, and must be used in conjunction with Compass Software version 2.5 or higher and compatible firmware. Bio-Techne uses cookies to provide you with a great website experience. Jess - Chemiluminescent & Fluorescent Western Blotting | ProteinSimple | Bio-Techne - IDO Recombinant Polyclonal Antibody (711778) All Rights Reserved. Jess gives you four ways to analyze proteins. Jess allows me to accurately assess drug compound potency, and with its high throughput ability I can screen multiple compounds quickly and efficiently. Jess automates the protein separation and immunodetection of traditional Western blotting, eliminating many of the tedious, error-prone steps. USA, TEL 612 379 2956 Postdoctoral Researcher, CSU Fullerton, "[Wes] Provides quantitative data, saves time and uses far less sample. USA, TEL 612 379 2956 ", Melyssa Bratton, Ph.D., WES protein simple system vs traditional Western blotting? ", Elena Navarro-Guerrero, Ph.D., Just load your samples and reagents into the microplate and Jess does the rest. PROTEINSIMPLE JESS USER MANUAL Pdf Download | ManualsLib Her proprietary fluorescent protein normalization reagent measures proteins immobilized in the same capillary as your immunoassay. Just load your samples and reagents into the microplate and Jess separates your proteins by size, and precisely manages antibody additions, incubations, washes and even the detection steps. 18 Share 2.8K views 2 years ago Watch this video to learn about WES and JESS - two highly reproducible, capillary based, automated western blot instruments by ProteinSimple. Senior Researcher, EURAC Research, "I work for a fast-paced drug discovery CRO where our clients expect high-quality data with rapid turnarounds. WES protein simple system vs traditional Western blotting? Simple Western immunoassays take place in a capillary. From the File menu, click New Assay and select the template assay for your instrument by selecting the assay type, the size range and cartridge type (if running Jess, Abby, or Wes) or choose Open Assay to ", Debbie Hicks, Ph.D., Automation of protein separation and immunodetection eliminates many of the tedious, error-prone steps of traditional Western blotting which limit data quality. Go further with high sensitivitybest in class fluorescence detection gets all the information you need in one shot. Software has many useful features for data analysis. Next, your sample is loaded, and capillaries are lowered to make contact with running buffer. For fluorescent detection, samples are incubated with the primary antibody followed by infrared or near-infrared fluorescent secondary tagged antibodies. Validation was considered when differential expression at one or more . With Jess's chemiluminescent detection, you'll get picogram level sensitivity letting you maximize the data you get from your sample. ", Nageswara Rao Boggavarapu, PhD, Supplier: ProteinSimple RP001. Scientist, Vividion Therapeutics, "I used Simple Western because the protein samples I had were small. ProteinSimple branded instruments are available to quote. The throughput and system automation is a definite advantage. View all citations for the Simple Western platform. Stellar NIR / IR modules for Jess set the standard for fluorescence Western blotting detection sensitivity. Early Development Scientist, Oxford Biomedica, "Jess allows us to immediately identify potential autophagy biomarkers as they are upregulated or downregulated in response to treatment. Insert a capillary cartridge into the cartridge holder. Bio-Techne Brands Which Brands are Currently Available on biotechne.com? PDF YOUR COMPLETE PROTEIN ANALYSIS SOLUTION - Biomed Global To gain the most data out of your precious sample, Jess provides a two-step immunoassay that is performed within the same capillary. USA, TEL 612 379 2956 Jess automates all steps of the process for you including sample loading, protein separation, immunoprobing or labeling, washing, detection and data analysis. Sample preparation, separation, and immobilization for theChemiluminescent Total Protein Assayare exactly the same asSimple Western automated immunoassays. ", Dr. Michael Harte, Snap! Help scientists and fund you research. Analyze entire signaling pathways with high sensitivity multiplexing and total protein normalization. Download the Total Protein Assay for AAV Analysis App Note to learn more about the use of ultra-high sensitivity total protein assays for AAV analysis in the development of cell & gene therapies. Protein Simple- Jess. You just load your samples and wait for results, and in the meantime, you can dedicate yourself to other activities. Detection of Stat3 (green) and phospho-Stat3 (red) in K562 cell lysate. Custom Targeted Protein Degradation Services, Certificate of Analysis (COA) Finder Tool. Dr. Wei showcases how imaged cIEF separates an AAV drug . Copyright 2023 Bio-Techne. Question Asked 20th May, 2021 Ritishka Kapoor Florida International University Western blotting using #Jess #proteinsimple? WES and JESS: Automated Western Blotting - YouTube With Stellar modules, low abundance proteins can be detected, and multiple targets can be multiplexed in the same sample using multi-color fluorescence channels. Analyzing Proteins with Jess. Jess gives you the qualitative western blot data you are used to seeing. Upgrade to Jess or Abby to have all the functionality of Wes with additional features like RePlex and Stellar High Sensitivity NIR/IR Detection (Jess only). Copyright 2023 Bio-Techne. $141.48 / Each of 1. Or look at multiple targets and parameters simultaneously. Learn how various capillary electrophoresis techniques can be easily implemented along the development path to support gene therapy development. Snap! Manager of the Cell, Molecular, and Biostatistics Core, Xavier University of Louisiana, "Wes is easy to use, convenient, and gives answers quickly. Jess automates the protein separation and immunodetection of traditional Western blotting, eliminating many of the tedious, error-prone steps.
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